Zero to Genetic Engineering Hero Home Pack (Chapters 5-7)

Overview

Building off of Zero to Genetic Engineering Hero Pack (Chapters 1-4), this kit pack includes a series of advanced kits that leverage the skills and knowledge learned in previous chapters. Learners will continue towards more advanced subjects such as extracting proteins from the cells that they have engineered, expressing enzymes to catalyze chemical reactions, and controlling the expression of genes using chemicals, heat, and light. 

In addition to completing fun and engaging activities, further real-world lab skills such as microcentrifugation, culturing cells, inducing expression, and filter sterilization are practiced.

While the activities and learnings in Chapters 5-7 delve into more advanced genetic engineering concepts and skills, these kits build off of the knowledge and skills learned in early Zero to Hero chapters and therefore can be used by teachers and students with no life science research experience and do not require pipettes. As you will read in Chapter 2 of Zero to Genetic Engineering, to do some of these advanced kits an additional piece of lab equipment is required, a microcentrifuge.

The Home Pack has one (1) of each individual kit for the exercises in Chapters 5-7 of Zero to Genetic Engineering Hero.

What's Included?

Extract-it Kit (Chapter 5)

The natural next step after a student learns how to engineer cells with a plasmid and how the cell reads the DNA plasmid gene (transcription) is to learn how to extract the engineered proteins from the cells and from a Fundamental concepts standpoint, to learn the process that the cells use to read the RNA and create proteins (translation). The hands-on exercise of extracting the proteins the students have engineered is a great anchor point for the Fundamentals section of Chapter 5, Translation. In the hands-on exercise, the students use every skill they have learned in Chapters 1-4, including lysing cells, streaking cells, incubating cells, and engineering cells!

In addition to a DNA Playground Large, this kit requires the use of a microcentrifuge.

 

Smell-it Kit & Blue-it Kit (Chapter 6)

Now that the students have mastered the basic concepts of cell function and lab skills of genetic engineering, Chapter 6 dives deeper into the chemistry that makes cells function, as well as a very important topic of genetic engineering - using enzymes to cause chemical reactions. The Smell-it Kit enables students to first engineer bacteria with a plasmid that creates an enzyme and then culture those cells with a substrate added to the LB agar plates. The students marvel as their engineered cells transform the musky smelling substrate into an overripe sweet banana scent. The Blue-it Kit also includes the engineering of bacteria to produce an enzyme. With the Blue-it Kit, however, the students engineer the cells, extract the engineered enzyme and complete a chemical reaction in a tube. The enzyme will convert a white powder into a vivid blue chemical.

The Fundamentals of Chapter 6 use the hands-on exercises as anchor points and dive deeper into chemical reactions. An important part of chemical reactions is bonding, and so a light introduction to atoms, orbitals, and the four types of bonds are discussed. Ultimately the students leave with an understanding of not only what proteins are, but how they function.

One of each the Smell-it Kit and Blue-it Kit are included as individually packed kits.  In addition to a DNA Playground, the Blue-it Kit requires the use of a microcentrifuge.

 

Heat-it Kit, Induce-it Kit, and RGB Kit (Chapter 7)

With a good understanding of transcription (Chapter 4) and translation (Chapter 5) and the basic bonding used in cells (Chapter 6), students can move onto gene regulation. These three kits use three different environmental factors to control the expression of genes.

The Heat-it Kit includes a DNA plasmid with a gene that has a promoter that is responsive to temperature. At 30 °C the promoter is in an OFF state, however, at 37 °C and especially 42 °C, the promoter is able to activate expression of a colored protein. The Fundamentals section describes how the temperature sensitive promoter relates to the cells basic stress response, and “hi-jacks” that response.

The Induce-it Kit explores the world of using chemicals to manipulate gene expression. In this instance, the students first engineer cells with a DNA plasmid containing a color-expressing gene that is sensitive to the presence of a chemical. After culturing their engineered cells, the students add the chemical to spots on the Petri dish and over the course of a few hours, begin to see the expression of the color protein.

The RGB Kit includes the use of a strain of bacteria that is sensitive to light. Using the DNA Playground Large, which has an LED light in its incubator, the student chooses a color of light using the touchscreen, which causes the expression of different color pigments. Red LED light causes the cells to create red pigment. Green LED light causes the cells to make green pigment. Blue light causes the cells to produce blue pigment. In the Fundamentals section, the gene regulation relating to the green pigment is described in detail.

One of each of the Heat-it Kit, Induce-it Kit, and RGB Kit are included as individually packaged units. 

 

Microcentrifuge (optional add-on)

A microcentrifuge is an important piece of equipment that is used in every life science research laboratory in the world. A microcentrifuge is a small toaster oven sized device that you can put microcentrifuge tubes (1.5 mL lab tubes) into and then spin at high speeds. This process allows you to separate different things in your tubes, an important part of doing science. The microcentrifuge offered as an optional add-on to this kit pack can hold up to 12 microcentrifuge tubes at a time and spin at 15,000 RPM (~14,000 x g). It has a timer so you set a time, start the centrifuge and with the timer reaches zero, it will automatically stop spinning.

If you already have a microcentrifuge, select "0" microcentrifuges in the drop down.  

Shipping

Packs are shipped 1-5 days after you place your order. If you require yours for a specific date or event, please contact us prior to, or with your order # so that we can try to make it happen for you! ☺︎ 

The kit(s) must be refrigerated upon arrival for longer shelf life. 

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