Learn genetic engineering hands-on using the Engineer-it Kit. The end result is that you have grown and engineered bacteria to produce something for you -- such as a colorful bio-pigment. After you’ve completed the Engineer-it Kit, you can use our follow-on kits, such as the Keep-it Kit to save your work, or Plate Extract-it Kit to break open the cells and extract your engineered creation.
The Engineer-it Kit is used in Chapter 4 of Zero to Genetic Engineering Hero, and if you haven't already, you may consider starting with the Canvas Kit, a fun bioart kit to learn how to grow cells (Chapter 3 of Zero to Genetic Engineering Hero). Once you've grown cells, you should move on to the Engineer-it Kit to complete your first genetic engineering experiment!
A course on Udemy guides you through the Engineer-it Kit experience and provides confidence, context and theory. Add it now for 25% off!
Learn about Zero to Genetic Engineering Hero, the worlds first beginner's guide to programming biology!
Individual kit- One try, can be done by individuals, groups, family. Best results using a DNA Playground (see the DNA Playground Starter Set)
Group kit -Eight genetic engineering tries with 1 shared DNA program - can be done individually or in groups up to 4.
Transformation Buffer: Amino Labs’ proprietary transformation buffer is used in a colony transformation procedure to yield high transformation efficiencies. When you strictly adhere to the transformation protocol, this buffer rivals or is better than other commercially available competent cell & procedures.
Recovery Media: Amino Labs' recovery media is used immediately after the heat shock during the transformation protocol. This nutrient broth aids the cells in recovering and has a proprietary recipe that further boosts the cells ability to survive the transformation and begin dividing.
Agar Powder: This LB agar powder is industry standard to grow microorganisms.
Cells: This standard K12 strain of E. coli is non-pathogenic and is the prototypical strain used by thousands of labs around the world.
DNA Program: This DNA plasmid is a small circular piece of DNA that programs the E. coli bacteria to create a colorful pigment that comes from ocean creatures. The pigments may be visible under normal light, but some also glow vibrantly under a black light.
Antibiotics for Transformation: Amino Labs' proprietary antibiotic delivery system helps stabilize antibiotics for shipping and long-term storage. These capsules have a measured amount of antibiotics for 45 mL of molten LB agar.
Sterile Water: Sterility is critical when you’re doing biotechnology. This is distilled water that has been sterilized with an autoclave in order to ensure there are no contaminating organisms present.
Loops: Small inoculating loops are used for transferring 1 uL of liquid and/or other tasks. Large inoculating loops are used for transferring 10 uL of liquid and/or other tasks. Large loops are great for spreading out bacteria after a transformation.
Petri Dish / Plate: 6cm Petri dishes are large enough for typical lab experiments and help save on the cost of reagents as well as reduce waste.
Engineer-it Kits are a general experience that can be used as a hands-on activity to complement various modules from middle school to high school. They allow for scaffolded learning and Engineer-it Kits are also used in technical colleges and universities.
Self-contained kit: No need to source and buy reagents, chemicals, and consumables. The Engineer-it Kit is a convenient collection of all that is needed to engineer and grow cells in Petri dishes.
All reagents tubes are color-coded for ease of use and include illustrated, simple procedures.
2 convenient formats:
Individual kit - One try, can be done by individuals, groups, family.
Group kit - Eight tries with 1 shared DNA program - can be done individually or in groups up to 4.
Choose from one of 9 pigments :
Blue, Violet, Orange, Teal, Purple
Fluorescent: Cyan, Magenta, Yellow, Raspberry Red
All Engineer-it Kits are Biosafety Level 1 (BSL1), meaning they do not require any special containment in North America (you can do it at home, school, etc.). If you are outside of North America, some regulations may apply, please check with your local public health office. Learn more about safety.
Complements the Next Generation Science Standards (NGSS):MS-LS1-1, MS-LS1-5, MS-LS1-8, HS-LS1-1, HS-LS3-1, HS-LS4-4, HS-LS4-5
Wondering how it works?
Engineer-it Kits are shipped 1-5 days after you place your order. If you require yours for a specific date or event, please contact us prior to, or with your order # so that we can make it happen for you! ☺︎
The kit(s) must be refrigerated upon arrival for longer shelf life.